Cell kinetics of human tumors by in vitro bromodeoxyuridine labeling.
نویسندگان
چکیده
منابع مشابه
Cell Kinetics in Human Malignancies Studied with in Vivo Administration of Bromodeoxyuridine and Flow Cytometry1
Bromodeoxyuridine (BrdUrd) is a pyrimidine analogue which is incor porated into the DN A of proliferating cells. When in vivoBrdUrd infusion is coupled with Divariate flow cytomctry to measure cell BrdUrd incor poration and I)V\ content, both the percentage of DNA-synthesizing cells jBrdUrd-labeling index (LI)] and the DNA synthesis time (Ts) can be determined on the same tissue sample. From ex...
متن کاملCell kinetics in human malignancies studied with in vivo administration of bromodeoxyuridine and flow cytometry.
Bromodeoxyuridine (BrdUrd) is a pyrimidine analogue which is incorporated into the DNA of proliferating cells. When in vivo BrdUrd infusion is coupled with bivariate flow cytometry to measure cell BrdUrd incorporation and DNA content, both the percentage of DNA-synthesizing cells [BrdUrd-labeling index (LI)] and the DNA synthesis time (TS) can be determined on the same tissue sample. From exper...
متن کاملThe proliferative potential of human ependymomas measured by in situ bromodeoxyuridine labeling.
Twelve patients with ependymomas received a 30- to 60-minute intravenous infusion of bromodeoxyuridine (BrdU), 150 to 200 mg/m2 at surgery, to label tumor cells in the DNA synthesis phase. Labeled cells were detected in excised tumor specimens by indirect immunoperoxidase staining using anti-BrdU monoclonal antibody as the first antibody. The BrdU labeling index (LI, defined as the percentage o...
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Background: Possibility to trace-label albumin with isotopes results in information concerning its synthesis, breakdown, and distribution in the intra and extra cellular spaces. The iodination of albumin is a widespread procedure used in scientific studies. Bromine not only is more reactive and less expensive than iodine, but bonds more easily with many elements. Therefore, it could be a suitab...
متن کاملDevelopment of [201Tl](III)oxinate complex for in vitro cell labeling
Background: The incorporation of thallium-201 into 8-hydroxyquinoline was targeted for cell labeling due to interesting physical properties and wide availability of this nuclide as a single photon emission computed tomography (SPECT) radionuclide. Materials and Methods: Thallium-201 (T1/2=3.04 d) in Tl+ form was converted to Tl3+ cation in presence of O3/6M HCl and di-isopropyl ether, controlle...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1989
ISSN: 0022-1554,1551-5044
DOI: 10.1177/37.9.2768814